| SDC3

Syndecan-3 is a protein that is encoded by the SDC3 gene.The protein encoded by this gene belongs to the syndecan proteoglycan family. It may play a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. Allelic variants of this gene have been associated with obesity.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	40	20	10	5	2.5	1.25	0.62	0
OD	2.66	1.97	1.36	0.83	0.51	0.32	0.2	0.08
Corrected OD	2.58	1.89	1.28	0.75	0.43	0.24	0.12	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human SDC3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	97-108	103

 

| Linearity

Samples were spiked with high concentrations of Human SDC3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)
1 : 2	Range (%)	90-110
	Average (%)	96
1 : 4	Range (%)	105-115
	Average (%)	108